evaluation of dna damage in cyprinus carpio (l. 1758) exposedto malathion using single cell gel electrophoresis

نویسندگان

a. mashinchian moradi

department of marine biology, graduate school of marine science and technology, science and research branch, islamic azad university, tehran, iran h. mozdarani

department of medical genetics, faculty of medical sciences, tarbiat modares university, tehran, iran p. alidoust salimi

department of marine biology, graduate school of marine science and technology, science and research branch, islamic azad university, tehran, iran m. alidoust salimi

department of marine biology, graduate school of marine science and technology, science and research branch, islamic azad university, tehran, iran

چکیده

malathion (s-(1, 2-dicarboethoxyethyl) o, o-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. in spite of widely used, little information are available about dna damage in aquatic organisms. therefore, the present study carried out to investigation of dna damage induced by malathion in cyprinus carpio (pisces: cyprinidae) using single cell gel electrophoresis. the condition of experiment was determined in static system. the specimens were exposed to different non-lethal concentrations (0.5, 1.5 and 3 mgl-1) of the malathion for 96 hour (short-time exposed). our results showed, the specimens exposed to different concentrations of malathion exhibited significantly higher dna damage in their blood cells than the control sample (p<0.05). this study confirmed that the comet assay is useful method in determining genotoxicity of pesticides. fish can be used for biomonitoring of the genotoxic pollutants in aquatic environment. furthermore, dna strand breakage can be used as biomarker in ecotoxicological studies.

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منابع مشابه

Evaluation of DNA damage in Cyprinus carpio (L. 1758) exposedto malathion using Single Cell Gel Electrophoresis

Malathion (S-(1, 2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. In spite of widely used, little information are available about DNA damage in aquatic organisms. Therefore, the present study carried out to investigation of DNA damage induced by malathion in Cyprinus carpio (Pisces: ...

متن کامل

evaluation of dna damage in cyprinus carpio (l. 1758) exposedto malathion using single cell gel electrophoresis

malathion (s-(1, 2-dicarboethoxyethyl) o, o-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. in spite of widely used, little information are available about dna damage in aquatic organisms. therefore, the present study carried out to investigation of dna damage induced by malathion in cyprinus carpio (pisces: ...

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عنوان ژورنال:
international journal of marine science and engineering

جلد ۲، شماره ۳، صفحات ۱۸۵-۱۸۸

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